目的 探讨龙血竭总黄酮(Sanguis Draconis flavones,SDF)对D-半乳糖致衰老大鼠抗氧化及免疫调节作用。方法 将60只SD大鼠随机分为6组,分别为正常组、模型组、阳性组,SDF低、中、高剂量组。颈背部皮下注射5% D-半乳糖生理盐水溶液建立大鼠亚急性衰老模型,同时灌胃给予相应药物6周,给药期间每周称量1次大鼠体重;实验结束后测定大鼠胸腺和脾脏指数;同时测定大鼠血清中丙二醛(MDA)、蛋白质羰基(PC)、晚期糖基化终末产物(AGES)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的含量或活性;酶联免疫法(ELISA)测定脑组织中脂褐素(LF)、过氧化脂质(LPO)以及B型单胺氧化酶(MAO-B)含量或活性;透射电镜观察脑组织超微结构改变。结果 大鼠亚急性衰老模型复制成功,与模型组比较,SDF高、中剂量组能够明显升高大鼠胸腺、脾脏指数;SDF高剂量组还可明显降低大鼠血清中MDA、PC、AGES的含量并升高SOD、GSH-PX的活性;SDF高、中剂量组能够降低大鼠脑组织中LPO、LF含量及MAO-B的活性;同时SDF高、中剂量组可改善大鼠脑组织中神经元的退行性改变。结论 SDF具有较好的延缓衰老作用,其机制可能与抗氧化及影响神经介质有关。
Abstract
OBJECTIVE To investigate the antioxidant ability and immune adjustment function of Sanguis Draconis flavones (SDF) on D-galactose aging in rats.METHODS The healthy SD rats were randomly divided into 6 groups: blank control group, aging model group, positive control group, low-dose, medium-dose and high-dose of SDF groups. Aging model was induced by subcutaneously injecting rats with 5% D-galactose. Rats of each group received daily administration by gavage for 6 weeks and the rats were weighted once per week in experimental period. The thymus and spleen index were measured; meanwhile, the contents of MDA, PC, AGES and the activity of SOD, GSH-PX in serum were detected after the experiment. The contents of LF, LPO and MAO-B activity in brain tissue were detected by ELISA method; the ultrastructural changes of brain tissue were observed by transmission electron microscopy.RESULTS Compared with the normal group, rat subacute aging model was replicated successfully; high-dose and medium-dose of SDF can significantly increase the thymus and spleen index of rats. high-dose of SDF can significantly decrease the contents of MDA, PC, AGES and increase the activity of SOD, GSH-PX; high-dose and medium-dose of SDF can reduce the LF, LPO contents and MAO-B activity in brain tissue. In addition, high-dose and medium-dose of SDF can improve the degeneration of neurons in rat brain.CONCLUSION SDF has obvious anti-aging effect on aging rats, which may be related to the antioxidant and the influence of neurotransmitters.
关键词
龙血竭总黄酮 /
衰老 /
抗氧化 /
神经元退行性改变
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Key words
Sanguis Draconis flavones /
aging /
antioxidation /
neurodegenerative changes
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中图分类号：
R965
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参考文献
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脚注
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基金
安徽省自然科学基金面上项目资助(1608085MH161)
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